The effect of hormones on blood sugar

The effect of hormones on blood sugar

Experimental principle
• There are two types of hormones involved in blood glucose regulation: one is a hormone that lowers blood sugar, only one is insulin; the other is a hormone that raises blood sugar, such hormones: adrenaline, glucagon, adrenocortical hormone, Growth hormone and so on.
• Blood glucose levels can be measured with a blood glucose meter. The principle of a blood glucose meter is to measure blood sugar by measuring the amount of current produced by the reaction of glucose in the blood with glucose dehydrogenase in the test paper.
Purpose
• Master the effects of insulin, glucagon, adrenaline, glucocorticoids, thyroxine, and auxin on blood glucose levels by measuring blood glucose levels.
• Learn how to measure blood sugar
Materials and Methods
• Experimental animals: mice
• Equipment and medicines: a set of surgical instruments for mammals, 5 syringes (5ml), 8 boxes with lids, 2% sodium pentobarbital solution, saline, insulin solution, glucagon solution, 1: 10000 adrenaline solution, glucocorticoid injection, thyroxine injection, nuptropin growth hormone injection

Methods and steps
• 1. Random grouping Fourteen healthy mice with similar body weight and similar strains were randomly divided into A and B groups, each group numbered 1, 2, 3, 4, 5, 6, 7,;
• 2. Group A anesthesia treatment: A group of mice were injected with 2% sodium pentobarbital, 0.005ml/g at the end of the mice. After anesthesia, the animals were placed on the operating table and the abdominal cavity was opened on the right side of the xiphoid. , suture the wound;
• In group B, the same incision was made in the area where the mice in group A were operated, and then the wound was sutured. The blood glucose concentration of each mouse was measured (glucometer) and the experimental data was recorded.
• 4. Drug treatment After the mice were quiet, the mice in the two groups were intraperitoneally injected with 0.5 ml of normal saline; the mice in the second group were injected with 0.5 ml of glucagon, and the mice of the third group were injected with 0.5 ml of epinephrine; Mouse No. 4 was intraperitoneally injected with 10 U/10 g of insulin; No. 5 mice were intraperitoneally injected with 0.5 ml of glucocorticoid; No. 6 mice were intraperitoneally injected with thyroxine 0.5 ml; and No. 7 mice were intraperitoneally injected with 0.5 ml of growth hormone. Place the mouse in a covered box, measure the blood glucose once every 5 minutes with a blood glucose meter, and record the experimental data;
Precautions
• The mode of administration of this experiment is intravenous. It should pay attention to the correct gesture of capturing animals and the site of injection. At the same time, pay attention to the dosage of the drug, including the dosing of the drug and the quantification of the syringe. In addition, it is usually necessary after the injection of the drug. The reaction of the mouse was observed in a covered box. The reaction was not only affected by the dose but also related to the surrounding temperature. The higher the temperature, the higher the proportion of the reaction.
Experimental expected result
• The blood glucose concentration of the mice before use as a reference;
• The blood glucose concentration of group A mice was significantly lower than the reference concentration. The blood glucose levels of mice 2, 3, 5, 6, and 7 were significantly higher than the reference concentration, and the blood glucose concentration of mice 1 decreased slightly.
• The blood glucose concentration of group B mice gradually decreased and then returned to the reference concentration. The blood glucose levels of mice 2, 3, 5, 6, and 7 gradually increased and then returned to the reference concentration. The blood glucose concentration of mouse No. 1 did not change significantly.

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